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Distinctive twin indexes minimize for each-sample Value and sequence libraries with the best accuracy compared to other indexing approaches.

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With the i5 index, assess coloration harmony using the forward orientation for MiSeq operates and MiniSeq operates with swift reagents, and the reverse-complement orientation for all other instruments.

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Established B Index Adapters Consult with Index two (i5) Orientation for more information regarding how to enter i5 bases on the sample sheet in forward or reverse enhance orientation.

This enables significant quantities of libraries to become pooled and sequenced simultaneously for the duration of one operate. Compared to other indexing techniques, special dual indexing mitigates index hopping by filtering hopped reads.

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Library prep, enrichment, and probe panel reagents must be requested separately. Appropriate library prep kits:

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